uncleR

Overview

uncleR is an R package for parsing data exported from an Unchained Labs Uncle instrument for downstream processing of biologics stability data.

The consolidate_experiments function utilizes the core functions listed below to import Uncle data exports into a succinct and readily accessible data structure based on the hierarchical structure of the directories in which the data is stored. Experimental data for a single run from nanoDSF, SLS, and DLS summary and spectra files are organized into a unified dataframe and nested within a named list returned by the function according to which experiment (i.e. plate or screen) they belong to.

Core functions for summary and “bundle export” files:

• import_staticBundle imports nanoDSF and SLS spectra bundle
• import_dynamicBundle imports DLS spectra bundle files
• import_DLSsum() imports DLS summary data
• import_SLSsum() imports nanoDSF and SLS thermal ramp summary data
• get_meta imports metadata from an Excel document into a list of dataframes
• add_meta assigns metadata to an imported and parsed dataframe

Legacy functions for “complete export” files:

• import_DLSspec() imports correlation, intensity, or mass distribution DLS spectra
• import_SLSspec imports SLS spectra at a specified wavelength
• import_FLUORspec() imports nanoDSF spectra

Questioning:

• join_SLS_DLS joins SLS and DLS data together before metadata assignment

Installation

You can install the most stable version from GitHub with:

if (!require(devtools)) install.packages("devtools")
devtools::install_github("eric-hunt/uncleR@main")

..or the development version with:

if (!require(devtools)) install.packages("devtools")
devtools::install_github("eric-hunt/uncleR@dev")